Plant growth-promoting Burkholderia species isolated from annual ryegrass in Portuguese soils.
Identifieur interne : 000B52 ( Main/Exploration ); précédent : 000B51; suivant : 000B53Plant growth-promoting Burkholderia species isolated from annual ryegrass in Portuguese soils.
Auteurs : N. Castanheira [Portugal] ; A C Dourado [Portugal] ; S. Kruz [Portugal] ; P I L. Alves [Portugal] ; A I Delgado-Rodríguez [Portugal] ; I. Pais [Portugal] ; J. Semedo [Portugal] ; P. Scotti-Campos [Portugal] ; C. Sánchez [Portugal] ; N. Borges [Portugal] ; G. Carvalho [Portugal] ; M T Barreto Crespo [Portugal] ; P. Fareleira [Portugal]Source :
- Journal of applied microbiology [ 1365-2672 ] ; 2016.
Descripteurs français
- KwdFr :
- ARN ribosomique 16S (génétique), Acides indolacétiques (métabolisme), Burkholderia (classification), Burkholderia (génétique), Burkholderia (isolement et purification), Burkholderia (métabolisme), Données de séquences moléculaires (MeSH), Lolium (croissance et développement), Lolium (microbiologie), Microbiologie du sol (MeSH), Phosphates (métabolisme), Portugal (MeSH), Racines de plante (croissance et développement), Racines de plante (microbiologie), Sol (composition chimique).
- MESH :
- composition chimique : Burkholderia, Sol.
- croissance et développement : Lolium, Racines de plante.
- génétique : ARN ribosomique 16S, Burkholderia.
- isolement et purification : Burkholderia.
- microbiologie : Lolium, Racines de plante.
- métabolisme : Acides indolacétiques, Burkholderia, Phosphates.
- Données de séquences moléculaires, Microbiologie du sol, Portugal.
English descriptors
- KwdEn :
- Burkholderia (classification), Burkholderia (genetics), Burkholderia (isolation & purification), Burkholderia (metabolism), Indoleacetic Acids (metabolism), Lolium (growth & development), Lolium (microbiology), Molecular Sequence Data (MeSH), Phosphates (metabolism), Plant Roots (growth & development), Plant Roots (microbiology), Portugal (MeSH), RNA, Ribosomal, 16S (genetics), Soil (chemistry), Soil Microbiology (MeSH).
- MESH :
- chemical , chemistry : Soil.
- chemical , genetics : RNA, Ribosomal, 16S.
- chemical , metabolism : Indoleacetic Acids, Phosphates.
- classification : Burkholderia.
- genetics : Burkholderia.
- growth & development : Lolium, Plant Roots.
- isolation & purification : Burkholderia.
- metabolism : Burkholderia.
- microbiology : Lolium, Plant Roots.
- Molecular Sequence Data, Portugal, Soil Microbiology.
Abstract
AIMS
To search for culturable Burkholderia species associated with annual ryegrass in soils from natural pastures in Portugal, with plant growth-promoting effects.
METHODS AND RESULTS
Annual ryegrass seedlings were used to trap Burkholderia from two different soils in laboratory conditions. A combined approach using genomic fingerprinting and sequencing of 16S rRNA and recA genes resulted in the identification of Burkholderia strains belonging to the species Burkholderia graminis, Burkholderia fungorum and the Burkholderia cepacia complex. Most strains were able to solubilize mineral phosphate and to synthesize indole acetic acid; some of them could produce siderophores and antagonize the phytopathogenic oomycete, Phytophthora cinnamomi. A strain (G2Bd5) of B. graminis was selected for gnotobiotic plant inoculation experiments. The main effects were the stimulation of root growth and enhancement of leaf lipid synthesis and turnover. Fluorescence in situ hybridization and confocal laser microscopy evidenced that strain G2Bd5 is a rhizospheric and endophytic colonizer of annual ryegrass.
CONCLUSIONS
This work revealed that annual ryegrass can naturally associate with members of the genus Burkholderia. A novel plant growth promoting strain of B. graminis was obtained.
SIGNIFICANCE AND IMPACT OF THE STUDY
The novel strain belongs to the plant-associated Burkholderia cluster and is a promising candidate for exploitation as plant inoculant in field conditions.
DOI: 10.1111/jam.13025
PubMed: 26671760
Affiliations:
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Scotti-Campos</name><affiliation wicri:level="1"><nlm:affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Sanchez, C" sort="Sanchez, C" uniqKey="Sanchez C" first="C" last="Sánchez">C. Sánchez</name><affiliation wicri:level="1"><nlm:affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Borges, N" sort="Borges, N" uniqKey="Borges N" first="N" last="Borges">N. 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Carvalho</name><affiliation wicri:level="1"><nlm:affiliation>UCBIO, REQUIMTE, Department of Chemistry, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCBIO, REQUIMTE, Department of Chemistry, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica</wicri:regionArea><wicri:noRegion>Caparica</wicri:noRegion></affiliation></author><author><name sortKey="Barreto Crespo, M T" sort="Barreto Crespo, M T" uniqKey="Barreto Crespo M" first="M T" last="Barreto Crespo">M T Barreto Crespo</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Fareleira, P" sort="Fareleira, P" uniqKey="Fareleira P" first="P" last="Fareleira">P. Fareleira</name><affiliation wicri:level="1"><nlm:affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26671760</idno><idno type="pmid">26671760</idno><idno type="doi">10.1111/jam.13025</idno><idno type="wicri:Area/Main/Corpus">000D10</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000D10</idno><idno type="wicri:Area/Main/Curation">000D10</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000D10</idno><idno type="wicri:Area/Main/Exploration">000D10</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Plant growth-promoting Burkholderia species isolated from annual ryegrass in Portuguese soils.</title><author><name sortKey="Castanheira, N" sort="Castanheira, N" uniqKey="Castanheira N" first="N" last="Castanheira">N. Castanheira</name><affiliation wicri:level="1"><nlm:affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Dourado, A C" sort="Dourado, A C" uniqKey="Dourado A" first="A C" last="Dourado">A C Dourado</name><affiliation wicri:level="1"><nlm:affiliation>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Kruz, S" sort="Kruz, S" uniqKey="Kruz S" first="S" last="Kruz">S. Kruz</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Alves, P I L" sort="Alves, P I L" uniqKey="Alves P" first="P I L" last="Alves">P I L. Alves</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Delgado Rodriguez, A I" sort="Delgado Rodriguez, A I" uniqKey="Delgado Rodriguez A" first="A I" last="Delgado-Rodríguez">A I Delgado-Rodríguez</name><affiliation wicri:level="1"><nlm:affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Pais, I" sort="Pais, I" uniqKey="Pais I" first="I" last="Pais">I. Pais</name><affiliation wicri:level="1"><nlm:affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Semedo, J" sort="Semedo, J" uniqKey="Semedo J" first="J" last="Semedo">J. Semedo</name><affiliation wicri:level="1"><nlm:affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Scotti Campos, P" sort="Scotti Campos, P" uniqKey="Scotti Campos P" first="P" last="Scotti-Campos">P. Scotti-Campos</name><affiliation wicri:level="1"><nlm:affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Sanchez, C" sort="Sanchez, C" uniqKey="Sanchez C" first="C" last="Sánchez">C. Sánchez</name><affiliation wicri:level="1"><nlm:affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Borges, N" sort="Borges, N" uniqKey="Borges N" first="N" last="Borges">N. Borges</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Carvalho, G" sort="Carvalho, G" uniqKey="Carvalho G" first="G" last="Carvalho">G. Carvalho</name><affiliation wicri:level="1"><nlm:affiliation>UCBIO, REQUIMTE, Department of Chemistry, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCBIO, REQUIMTE, Department of Chemistry, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica</wicri:regionArea><wicri:noRegion>Caparica</wicri:noRegion></affiliation></author><author><name sortKey="Barreto Crespo, M T" sort="Barreto Crespo, M T" uniqKey="Barreto Crespo M" first="M T" last="Barreto Crespo">M T Barreto Crespo</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Fareleira, P" sort="Fareleira, P" uniqKey="Fareleira P" first="P" last="Fareleira">P. Fareleira</name><affiliation wicri:level="1"><nlm:affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras</wicri:regionArea><wicri:noRegion>Oeiras</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of applied microbiology</title><idno type="eISSN">1365-2672</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Burkholderia (classification)</term><term>Burkholderia (genetics)</term><term>Burkholderia (isolation & purification)</term><term>Burkholderia (metabolism)</term><term>Indoleacetic Acids (metabolism)</term><term>Lolium (growth & development)</term><term>Lolium (microbiology)</term><term>Molecular Sequence Data (MeSH)</term><term>Phosphates (metabolism)</term><term>Plant Roots (growth & development)</term><term>Plant Roots (microbiology)</term><term>Portugal (MeSH)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Soil (chemistry)</term><term>Soil Microbiology (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ribosomique 16S (génétique)</term><term>Acides indolacétiques (métabolisme)</term><term>Burkholderia (classification)</term><term>Burkholderia (génétique)</term><term>Burkholderia (isolement et purification)</term><term>Burkholderia (métabolisme)</term><term>Données de séquences moléculaires (MeSH)</term><term>Lolium (croissance et développement)</term><term>Lolium (microbiologie)</term><term>Microbiologie du sol (MeSH)</term><term>Phosphates (métabolisme)</term><term>Portugal (MeSH)</term><term>Racines de plante (croissance et développement)</term><term>Racines de plante (microbiologie)</term><term>Sol (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Soil</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Indoleacetic Acids</term><term>Phosphates</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Burkholderia</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Burkholderia</term><term>Sol</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Lolium</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Burkholderia</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Lolium</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN ribosomique 16S</term><term>Burkholderia</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Burkholderia</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Burkholderia</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Burkholderia</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lolium</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lolium</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides indolacétiques</term><term>Burkholderia</term><term>Phosphates</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Sequence Data</term><term>Portugal</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Microbiologie du sol</term><term>Portugal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>AIMS</b></p><p>To search for culturable Burkholderia species associated with annual ryegrass in soils from natural pastures in Portugal, with plant growth-promoting effects.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS AND RESULTS</b></p><p>Annual ryegrass seedlings were used to trap Burkholderia from two different soils in laboratory conditions. A combined approach using genomic fingerprinting and sequencing of 16S rRNA and recA genes resulted in the identification of Burkholderia strains belonging to the species Burkholderia graminis, Burkholderia fungorum and the Burkholderia cepacia complex. Most strains were able to solubilize mineral phosphate and to synthesize indole acetic acid; some of them could produce siderophores and antagonize the phytopathogenic oomycete, Phytophthora cinnamomi. A strain (G2Bd5) of B. graminis was selected for gnotobiotic plant inoculation experiments. The main effects were the stimulation of root growth and enhancement of leaf lipid synthesis and turnover. Fluorescence in situ hybridization and confocal laser microscopy evidenced that strain G2Bd5 is a rhizospheric and endophytic colonizer of annual ryegrass.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>This work revealed that annual ryegrass can naturally associate with members of the genus Burkholderia. A novel plant growth promoting strain of B. graminis was obtained.</p></div><div type="abstract" xml:lang="en"><p><b>SIGNIFICANCE AND IMPACT OF THE STUDY</b></p><p>The novel strain belongs to the plant-associated Burkholderia cluster and is a promising candidate for exploitation as plant inoculant in field conditions.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26671760</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-2672</ISSN><JournalIssue CitedMedium="Internet"><Volume>120</Volume><Issue>3</Issue><PubDate><Year>2016</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of applied microbiology</Title><ISOAbbreviation>J Appl Microbiol</ISOAbbreviation></Journal><ArticleTitle>Plant growth-promoting Burkholderia species isolated from annual ryegrass in Portuguese soils.</ArticleTitle><Pagination><MedlinePgn>724-39</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/jam.13025</ELocationID><Abstract><AbstractText Label="AIMS" NlmCategory="OBJECTIVE">To search for culturable Burkholderia species associated with annual ryegrass in soils from natural pastures in Portugal, with plant growth-promoting effects.</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">Annual ryegrass seedlings were used to trap Burkholderia from two different soils in laboratory conditions. A combined approach using genomic fingerprinting and sequencing of 16S rRNA and recA genes resulted in the identification of Burkholderia strains belonging to the species Burkholderia graminis, Burkholderia fungorum and the Burkholderia cepacia complex. Most strains were able to solubilize mineral phosphate and to synthesize indole acetic acid; some of them could produce siderophores and antagonize the phytopathogenic oomycete, Phytophthora cinnamomi. A strain (G2Bd5) of B. graminis was selected for gnotobiotic plant inoculation experiments. The main effects were the stimulation of root growth and enhancement of leaf lipid synthesis and turnover. Fluorescence in situ hybridization and confocal laser microscopy evidenced that strain G2Bd5 is a rhizospheric and endophytic colonizer of annual ryegrass.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">This work revealed that annual ryegrass can naturally associate with members of the genus Burkholderia. A novel plant growth promoting strain of B. graminis was obtained.</AbstractText><AbstractText Label="SIGNIFICANCE AND IMPACT OF THE STUDY" NlmCategory="CONCLUSIONS">The novel strain belongs to the plant-associated Burkholderia cluster and is a promising candidate for exploitation as plant inoculant in field conditions.</AbstractText><CopyrightInformation>© 2015 The Society for Applied Microbiology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Castanheira</LastName><ForeName>N</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dourado</LastName><ForeName>A C</ForeName><Initials>AC</Initials><AffiliationInfo><Affiliation>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kruz</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alves</LastName><ForeName>P I L</ForeName><Initials>PI</Initials><AffiliationInfo><Affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Delgado-Rodríguez</LastName><ForeName>A I</ForeName><Initials>AI</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pais</LastName><ForeName>I</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Semedo</LastName><ForeName>J</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scotti-Campos</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sánchez</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Borges</LastName><ForeName>N</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carvalho</LastName><ForeName>G</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>UCBIO, REQUIMTE, Department of Chemistry, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barreto Crespo</LastName><ForeName>M T</ForeName><Initials>MT</Initials><AffiliationInfo><Affiliation>Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fareleira</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>KT737804</AccessionNumber><AccessionNumber>KT737814</AccessionNumber><AccessionNumber>KT737815</AccessionNumber><AccessionNumber>KT737820</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Appl Microbiol</MedlineTA><NlmUniqueID>9706280</NlmUniqueID><ISSNLinking>1364-5072</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007210">Indoleacetic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010710">Phosphates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012987">Soil</NameOfSubstance></Chemical><Chemical><RegistryNumber>6U1S09C61L</RegistryNumber><NameOfSubstance UI="C030737">indoleacetic acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019117" MajorTopicYN="N">Burkholderia</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007210" MajorTopicYN="N">Indoleacetic Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008129" MajorTopicYN="N">Lolium</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010710" MajorTopicYN="N">Phosphates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011174" MajorTopicYN="N">Portugal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012987" MajorTopicYN="N">Soil</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Burkholderia graminis</Keyword><Keyword MajorTopicYN="N">fluorescence in situ hybridization</Keyword><Keyword MajorTopicYN="N">plant growth-promoting activities</Keyword><Keyword MajorTopicYN="N">plant inoculation assays</Keyword><Keyword MajorTopicYN="N">plant-associated bacteria</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>09</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>11</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>12</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26671760</ArticleId><ArticleId IdType="doi">10.1111/jam.13025</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Portugal</li></country></list><tree><country name="Portugal"><noRegion><name sortKey="Castanheira, N" sort="Castanheira, N" uniqKey="Castanheira N" first="N" last="Castanheira">N. Castanheira</name></noRegion><name sortKey="Alves, P I L" sort="Alves, P I L" uniqKey="Alves P" first="P I L" last="Alves">P I L. Alves</name><name sortKey="Alves, P I L" sort="Alves, P I L" uniqKey="Alves P" first="P I L" last="Alves">P I L. Alves</name><name sortKey="Barreto Crespo, M T" sort="Barreto Crespo, M T" uniqKey="Barreto Crespo M" first="M T" last="Barreto Crespo">M T Barreto Crespo</name><name sortKey="Barreto Crespo, M T" sort="Barreto Crespo, M T" uniqKey="Barreto Crespo M" first="M T" last="Barreto Crespo">M T Barreto Crespo</name><name sortKey="Borges, N" sort="Borges, N" uniqKey="Borges N" first="N" last="Borges">N. Borges</name><name sortKey="Carvalho, G" sort="Carvalho, G" uniqKey="Carvalho G" first="G" last="Carvalho">G. Carvalho</name><name sortKey="Castanheira, N" sort="Castanheira, N" uniqKey="Castanheira N" first="N" last="Castanheira">N. Castanheira</name><name sortKey="Delgado Rodriguez, A I" sort="Delgado Rodriguez, A I" uniqKey="Delgado Rodriguez A" first="A I" last="Delgado-Rodríguez">A I Delgado-Rodríguez</name><name sortKey="Dourado, A C" sort="Dourado, A C" uniqKey="Dourado A" first="A C" last="Dourado">A C Dourado</name><name sortKey="Fareleira, P" sort="Fareleira, P" uniqKey="Fareleira P" first="P" last="Fareleira">P. Fareleira</name><name sortKey="Kruz, S" sort="Kruz, S" uniqKey="Kruz S" first="S" last="Kruz">S. Kruz</name><name sortKey="Kruz, S" sort="Kruz, S" uniqKey="Kruz S" first="S" last="Kruz">S. Kruz</name><name sortKey="Pais, I" sort="Pais, I" uniqKey="Pais I" first="I" last="Pais">I. Pais</name><name sortKey="Sanchez, C" sort="Sanchez, C" uniqKey="Sanchez C" first="C" last="Sánchez">C. Sánchez</name><name sortKey="Scotti Campos, P" sort="Scotti Campos, P" uniqKey="Scotti Campos P" first="P" last="Scotti-Campos">P. 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